5.36 | Spring 2009 | Undergraduate

Biochemistry Laboratory

Lecture Notes

Notes and slides are available for selected lectures below.

SES # TOPICS LECTURE NOTES
Lec 1 Introduction. Kinases in healthy and cancerous cells

Lecture 1 handout (PDF)

Lecture 1 slides (PDF - 4.7 MB)

Lec 2 DNA digestion and PCR

Lecture 2 handout (PDF)

Lecture 2 slides (PDF - 2.3 MB)

Lec 3 Affinity tags for protein purification/detection

Lecture 3 handout (PDF)

Lecture 3 slides (PDF - 1.2 MB)

Lec 4 Conserved and variable structural features of kinase domains

Lecture 4 handout (PDF)

Lecture 4 slides (PDF - 1.8 MB)

Lec 5 Site directed mutagenesis (Quikchange method) Lecture 5 handout (PDF)
Lec 6 Detecting kinase activity

Lecture 6 handout (PDF)

Lecture 6 slides (PDF - 2.8 MB)

Course Info

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Spring 2009
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Lecture Notes