This note covers Common laboratory tools and
equipment used in biochemistry and molecular biology, Units, solutions,
dialysis, Acid base equilibria, pH, buffer systems,Spectrophotometry
and protein concentration measurements, Cell disruption, cell fractionation and
protein isolation, Chromatographic methods, Electrophoresis, Protein ligand
interactions, Enzyme kinetics, Recombinant DNA technology, Bioinformatics,
Calculations and problem solving exercises.
The book deals with basic issues and
some of the recent developments in biochemistry. Particular emphasis is devoted
to both theoretical and experimental aspect of modern biochemistry.
Medical biochemistry is an essential component of curriculum for all
categories of health professionals. This lecture note on Medical biochemistry
integrates and summarizes the essentials of the core subject. Topics covered
includes: Conformation of biomolecules, structure and their relationship to
biological activity, synthesis and degradation of major metabolites, Production
and storage of energy, Biocatalysts and their application, Intercellular
communication by hormones, Molecular events in gene expression and regulation.
Author(s): The Carter Center, The Ethiopia Ministry
of Health