This
multi-topic resource covers key spectroscopy techniques used in the analysis of
molecular and atomic systems. The note encompasses a detailed introduction to
electronic spectroscopy, IR spectroscopy, and NMR spectroscopy, including both
proton and carbon types, mass spectrometry, electron spin resonance (ESR), and
Mössbauer spectroscopy. There is also a detailed description of every technique
in its basic principles, experimental setup, and applications in different
fields like chemistry, materials science, and biochemistry. The note also
focuses on how these spectroscopic methods are applied to understand molecular
structures, chemical bonding, and dynamic processes. Perfect for students and
researchers, the book provides both a theoretical background and practical
insights into the more general applications of spectroscopy in modern scientific
research.
Author(s): Sathyabama Institute of Science and Technology
This note aims to have a detailed
explanation of Nuclear Magnetic Resonance (NMR) spectroscopy focusing on its
applications in the study of molecular structure, especially proteins and other
biomolecules. Key topics explored include the electromagnetic spectrum, the
vector model of NMR, magnetization properties, and NMR excitation. The note
further introduces advanced concepts like chemical shift, nuclear shielding, and
spin-spin coupling as all forms necessary for interpreting NMR spectra. There
have been introductions of multi-dimensional NMR techniques that use them in
protein structure determination, and this book is very useful for researchers
and students to understand the theoretical and applied aspects of NMR
spectroscopy.
This note
focuses on how spectroscopic techniques can be integrated to determine the
structure of unknown molecules. It starts off with an overview of the
electromagnetic spectrum and basic 1H NMR, moving on to much advanced discussion
about how NMR can be combined with other methods such as IR, UV, and mass
spectrometry for an overall structural analysis. It goes over chemical shifts of
protons, coupling constants, and the use of 2D NMR for obtaining detailed
structural information. Other topics discussed include multinuclear NMR, dynamic
NMR and conformational, as well as chemical exchange rates measurement. This
note is perfect for students and researchers who want to use several
spectroscopic methods in the determination of the structure of a compound.