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Basics of Spectroscopy by UCF
This PDF covers the following topics related to Spectroscopy : Introduction, Prerequisites, Objectives, Scenario, Basic Concepts, Introduction, A Brief History of Spectroscopy, Creating Electromagnetic (EM) Waves, The Electromagnetic Spectrum, Particle Properties of Electromagnetic Energy, Energy Levels and Photons, Spectra of Light Sources, Regions of the Optical Spectrum, Emission and Absorption Spectra, Emission spectra, Absorption spectra, Fluorescence and Phosphorescence, Spectroscopic Instruments, Prism spectrometer, Diffraction grating, Interferometer spectrometer, Applications of Spectroscopy, Laboratory, Problem Exercises, Resources.
Author(s): OP-TEC, The National Center for Optics and Photonics Education
34 Pages 
This note aims to have a detailed explanation of Nuclear Magnetic Resonance (NMR) spectroscopy focusing on its applications in the study of molecular structure, especially proteins and other biomolecules. Key topics explored include the electromagnetic spectrum, the vector model of NMR, magnetization properties, and NMR excitation. The note further introduces advanced concepts like chemical shift, nuclear shielding, and spin-spin coupling as all forms necessary for interpreting NMR spectra. There have been introductions of multi-dimensional NMR techniques that use them in protein structure determination, and this book is very useful for researchers and students to understand the theoretical and applied aspects of NMR spectroscopy.
Author(s): David Case
124 Pages
Structure Determination Using Spectroscopic Methods
This note focuses on how spectroscopic techniques can be integrated to determine the structure of unknown molecules. It starts off with an overview of the electromagnetic spectrum and basic 1H NMR, moving on to much advanced discussion about how NMR can be combined with other methods such as IR, UV, and mass spectrometry for an overall structural analysis. It goes over chemical shifts of protons, coupling constants, and the use of 2D NMR for obtaining detailed structural information. Other topics discussed include multinuclear NMR, dynamic NMR and conformational, as well as chemical exchange rates measurement. This note is perfect for students and researchers who want to use several spectroscopic methods in the determination of the structure of a compound.
Author(s): Hans J. Reich
NA Pages