In mass spectrometry, one generates ions from a
sample to be analyzed. These ions are then separated and quantitatively
detected. The units covered in this notes are as follows : Introduction, Charge
Location and Fragmentation, Ion Separation, Ionization and Desorption,
Biomolecules, Literature, Sources.
Author(s): Peter
M. van Galen and Martin C. Feiters, Department of Organic Chemistry, Molecular
Chemistry Cluster, Institute for Molecules and Materials, Faculty of Science,
Radboud University, Nijmegen
Prof.
Peter B. O'Connor's note on mass spectrometry. It deals with the interpretation
of mass spectra; it would be particularly useful for any persons undertaking the
analysis of unknown samples. It starts by dealing with a range of techniques,
among them being electron impact ionization, MS/MS fragmentation, and ion
sources. In addition, the note goes into practical implementations of mass
spectrometry in real-world applications such as protein complex analysis,
hydrogen and deuterium exchange, and imaging mass spectrometry. The course takes
the student through advanced topics like 2D FTICR mass spectrometry, an
expansive full view of modern mass spectrometric methods and their practical use
in research and industry.
This note gives
a fairly accessible introduction to the principles and applications of mass
spectrometry. It starts by outlining important topics, such as sample
introduction, ionization techniques (electron and chemical ionization), and mass
analyzers. It then discusses the different types of detectors and the vacuum
systems required for successful analysis. It offers a tutorial overview of how
mass spectrometry can be applied to the analysis of complex chemical systems,
including an introduction to data systems and interpretation techniques. This
note is perfect for newcomers in the analytical chemistry arena seeking to get a
good grasp of the basics of mass spectrometry.