This resource from the Scripps Center for Metabolomics
introduces the fundamental concepts of mass spectrometry (MS), so it is very
good for beginners. It includes the most essential facts about MS that concern
the principles of ionization, mass analyzers, and detectors, as well as
information on the vacuum system required for MS analysis. The note gives
foundational knowledge of how MS works: from the generation of ions from a
sample to the separation process and on to detection. Suitable for practitioners
who are just beginning a career in analytical chemistry or metabolomics, it
provides an easily accessible introduction to the technology and applications in
the analysis of complex molecular systems.
This book serves as an introduction to
tandem mass spectrometry (MS/MS) and its role in molecular characterization in
chemical and biological research areas. It is tailored to professionals and
students alike and assesses the application of MS/MS in studying complex
molecules such as drugs, metabolites, carbohydrates, and proteins. The authors
explain how tandem mass spectrometry can provide detailed structural information
and insights into post-translational modifications. This resource is ideal for
those looking to understand the powerful applications of MS/MS in molecular
biology and chemistry, with emphasis on its versatility in biological and
pharmaceutical sciences.
Author(s): Ana Varela Coelho
and Catarina de Matos Ferraz Franco
This note presents
an overview of mass spectrometry at an advanced level with specific emphasis on
its utility in chemistry and biochemistry. Fundamental concepts include
ionization, mass analyzers, and dissociation of ions, as well as more advanced
topics like ion mobility and fragmentation. The qualitative and quantitative
analysis using mass spectrometry and the practical applications in various
fields are also considered. This note is designed for advanced students and
professionals aiming to gain insights into the role of mass spectrometry as an
analytical tool, both in theory and practice.