This note presents
an overview of mass spectrometry at an advanced level with specific emphasis on
its utility in chemistry and biochemistry. Fundamental concepts include
ionization, mass analyzers, and dissociation of ions, as well as more advanced
topics like ion mobility and fragmentation. The qualitative and quantitative
analysis using mass spectrometry and the practical applications in various
fields are also considered. This note is designed for advanced students and
professionals aiming to gain insights into the role of mass spectrometry as an
analytical tool, both in theory and practice.
This is a comprehensive note on
X-ray spectroscopy techniques, aimed at detailing their applications in
different fields, with an emphasis on the special applications in nanoparticles
and molecular mechanisms. It begins with the introduction of XRF processes and
techniques followed by high-resolution X-ray spectroscopy using compound
semiconductor detectors and digital pulse processing systems. It also includes
analytic applications of X-ray techniques, including dispersive X-ray
spectroscopy as well as quantification in X-ray fluorescence spectrometry. In
addition, it approaches specialized uses such as time-resolved X-ray absorption
spectroscopy in the study of metalloproteins and the application of soft X-rays
in astrochemistry. The note concludes discussing nanoscale chemical analysis
that highlights energy dispersive X-ray spectroscopy (EDX) and transmission
electron microscopy (TEM) with relevance to the characterization of interfaces
and materials on the nanoscale.
This book serves as an introduction to
tandem mass spectrometry (MS/MS) and its role in molecular characterization in
chemical and biological research areas. It is tailored to professionals and
students alike and assesses the application of MS/MS in studying complex
molecules such as drugs, metabolites, carbohydrates, and proteins. The authors
explain how tandem mass spectrometry can provide detailed structural information
and insights into post-translational modifications. This resource is ideal for
those looking to understand the powerful applications of MS/MS in molecular
biology and chemistry, with emphasis on its versatility in biological and
pharmaceutical sciences.
Author(s): Ana Varela Coelho
and Catarina de Matos Ferraz Franco